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19-Nortestosterone ELISA KIT

19-Nortestosterone  ELISA Kit is intended for the quantitative determination of 19-Nortestosterone in foodstuff and food supplements.

It is possible to determine up to 42/18 samples in duplicates (96/48 wells microtiter plate) with the set 19-Nortestosterone ELISA Kit.

Time required for ELISA determination: 2 hours 10 min
Limit of detection (LOD) : 100 ppb
Limit of quantification (LOQ) : 400 ppb
Calibration scale range: 100 ďż˝ 50 000 ppb

Anabolic steroids are synthetic derivatives of testosterone. Certain clinical effects and adverse reactions demonstrate the androgenic properties of this class of drugs.  Complete dissociation of anabolic and androgenic effects has not been achieved.  The actions of anabolic steroids are therefore similar to those of male sex hormones with the possibility of causing serious disturbances of growth and sexual development if given to young children. 19-Nortestosterone is an anabolic steroid also used as a growth promoting agent in the production of meat. 19-Nortestosterone ELISA test kit provides screening for 19-Nortestosterone in a dietary supplements and growth promoter residues in tissue.

The determination of 19-nortestosterone is based on its immunochemical reaction with a specific antibody. 19-nortestosterone present in analyzed sample and 19-nortestosterone (marked with biotin) coated on the walls of wells react in the first step (incubation) with a specific antibody. 19-nortestosterone on a solid phase and 19-nortestosterone of the sample compete for access to binding spots of the antibody against 19-nortestosterone. After the wells are rinsed, the horse-radish peroxidase conjugated anti rabbit antibody is added. After incubation the wells are rinsed again and the peroxidase that is bonded to the walls of the wells is detected by the addition of a chromogenic substrate (tetramethylbenzidine). The intensity of thus obtained colouration is inversely proportional to the concentration of 19-nortestosterone in calibrators, control samples and analysed samples.