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Boldenone ELISA KIT

Boldenone  ELISA Kit is intended for the quantitative determination of Boldenone in foodstuff and food supplements.

It is possible to determine up to 42/18 samples in duplicates (96/48 wells microtiter plate) with the Boldenone ELISA Kit.

Time required for ELISA determination: 2 hours 10 min
Limit of detection (LOD) : 20 ppb
Limit of quantification (LOQ) : 85 ppb
Calibration scale range: 50 ďż˝ 50 000 ppb

Anabolic steroids are synthetic derivatives of testosterone. Certain clinical effects and adverse reactions demonstrate the androgenic properties of this class of drugs.  Complete dissociation of anabolic and androgenic effects has not been achieved.  The actions of anabolic steroids are therefore similar to those of male sex hormones with the possibility of causing serious disturbances of growth and sexual development if given to young children. Boldenone (BOL) is an anabolic steroid that differs from testosterone only by one double bond at the 1- position. It is used mainly as undecylenate ester by bodybuilders and is administered illegally to racing horses. However, it is used as a growth promotor on farms improving the growth and feed conversion of cattle; it may be abused to achieve more efficient meat production. Boldenone ELISA test kit provides screening for boldenone in a dietary supplements and growth promoter residues in tissue.

The determination of boldenone is based on its immunochemical reaction with a specific antibody. Boldenone present in analyzed sample and boldenone (marked with biotin) coated on the walls of wells react in the first step (incubation) with a specific antibody.

Boldenone on a solid phase and boldenone of the sample compete for access to binding spots of the antibody against boldenone. After the wells are rinsed, the horse-radish peroxidase conjugated anti rabbit antibody is added. After incubation the wells are rinsed again and the peroxidase that is bonded to the walls of the wells is detected by the addition of a chromogenic substrate (tetramethylbenzidine). The intensity of thus obtained colouration is inversely proportional to the concentration of boldenone in calibrators, control samples and analysed samples.