Bovine Serum Albumin ELISA Kit is intended for the quantitative determination of BSA in dairy foodstuff. This kit is not suitable for the determination of BSA in heat treated foodstuff (the temperature of the heat processing should not exceed 100 °C).
Bovine Serum Albumin ELISA Kit contains all the required reagents for extraction and quantitative determination of BSA. It is possible to determine up to 41/17 samples in duplicates (96 wells microtitre plate/48 wells microtitre plate) with the set Bovine Serum Albumin.
| Time required for the sample preparation and extraction: | 1 hour (10 samples) |
| Time required for ELISA determination: | 1 hour 10 min |
| Limit of detection (LOD): | 0.62 ppm (mg/kg) |
| Limit of quantification (LOQ): | 3.76 ppm (mg/kg) |
| Calibration scale range: | 3.5 – 300 ppm |
| Expiration time: | 12 months (at 2 – 8 °C) |
Bovine serum contains ca. 6% of bovine serum albumin (BSA). The BSA, having molecular weight of 66,430 Da, consists of 583 amino acids. Within the field of immunoanalysis, the BSA is a well known blocker, commonly used to inhibit non – specific sorptions of other proteins or peptides. Beef is a significant component of food for considerable part of world population. Prevalence of allergies to BSA is no doubt less than that to other food allergens (gluten, eggs, cow's milk, soya, nuts, etc.). However, specialist physicians – allergologists do recommend the determination of BSA as a foodstuff allergen. Not negligible is also the fact that the presence or absence of BSA in foodstuffs can be exploited as the parameter utilisable for assessment/evaluation of the specified foodstuff composition as well as of observance of the procedure specified for the foodstuff production. In both relevant cases of BSA determination - as the foodstuff allergen or unwanted contaminant of the foodstuff – the determination represents the analysis of analyte with concentration at the level of units to dozens of ppm. For this type of analysis, with so low a concentration of targeted BSA, at simultaneous presence of other proteins occurring in surplus of several orders of magnitude, the promising, self-offering method of analysis could be the determination utilising specific antibodies.
Immuno-enzymatic determination of BSA is based on application of a competition system. In the wells of microtitre plate, walls of which are coated with sheep anti - BSA antibody, the sample to be analysed, while combined with BSA conjugate, is homogenised with horse - radish peroxidase. During the step of subsequent incubation, BSA will be bound to the wells walls. BSA present in the sample and BSA bound in the conjugate will compete mutually for access to the binding sites present in limited number in the antibody against BSA. After subsequent incubation step, the wells are washed and the peroxidase bound to the wells walls is detected by means of a chromogenic substrate (TMB), added to the system. Intensity of thus developed colouration is inversely proportional to BSA concentration in calibrators and analysed samples.
