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Stanazolol ELISA KIT

Stanazolol  ELISA Kit is intended for the quantitative determination of Stanazolol in foodstuff and food supplements.

It is possible to determine up to 42/18 samples in duplicates (96/48 wells microtiter plate) with the Stanazolol ELISA Kit.

Time required for ELISA determination: 2 hours 10 min

Anabolic steroids are synthetic derivatives of testosterone. Certain clinical effects and adverse reactions demonstrate the androgenic properties of this class of drugs.  Complete dissociation of anabolic and androgenic effects has not been achieved.  The actions of anabolic steroids are therefore similar to those of male sex hormones with the possibility of causing serious disturbances of growth and sexual development if given to young children. Stanazolol is a 17α-alkylatedandrostane steroid and a derivative of 5α-dihydrotestosterone (DHT). It is used perorally by bodybuilders and is administered illegally to racing horses. Stanozolol was used in both animals and human patients for a number of conditions. In humans, it has been demonstrated to be successful in treating anaemia and hereditary angioedema, but its use is strictly controlled in most countries.Veterinarians may prescribe the drug to improve muscle growth, red blood cell production, increase bone density and stimulate the appetite of debilitated or weakened animals. Stanazolol ELISA test kit provides screening for stanazolol in a dietary supplements and growth promoter residues in tissue.

The determination of Stanazolol is based on its immunochemical reaction with a specific antibody. Stanazolol present in analyzed sample and Stanazolol (marked with biotin) coated on the walls of wells react in the first step (incubation) with a specific antibody.

Stanazolol on a solid phase and Stanazolol of the sample compete for access to binding spots of the antibody against Stanazolol . After the wells are rinsed, the horse-radish peroxidase conjugated anti rabbit antibody is added. After incubation the wells are rinsed again and the peroxidase that is bonded to the walls of the wells is detected by the addition of a chromogenic substrate (tetramethylbenzidine). The intensity of thus obtained colouration is inversely proportional to the concentration of Stanazolol in calibrators, control samples and analysed samples.