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TSH ELISA Kit is intended for the quantitative determination of thyrotropin (TSH) in bovine and ovine serum.

It is possible to determine up to 41/17 samples in duplicates (96/48 wells microtiter plate) with the set TSH ELISA Kit.

Time required for ELISA determination: 2 hours 10 min
Limit of detection (LOD): 0.225 ng/ml
Limit of quantification: (LOQ) 0.5 ng/ml
Calibration scale range: 0.5 – 40 ng/ml

Thyroid-stimulating hormone (TSH; thyrotropin) and TSH receptor (TSHR) are key proteins in the control of thyroid function. TSH synthesis in the anterior pituitary is stimulated by thyrotropin-releasing hormone (TRH) and inhibited by thyroid hormone in a classical endocrine negative-feedback loop. TSH controls thyroid function upon its interaction with the G protein-coupled TSHR.

Thyroidal hormones crucial affect the whole metabolism of organism. Iodine intake from the environment is the critical factor, which influences function of thyroid gland. The height of the follicular cells, size of the follicles and amount and character of the colloid inside the follicles all depend on the activity of the thyroid.

The determination of thyrotropin (TSH) is based on its immunochemical reaction bovine/ ovine TSH with a specific antibody. TSH present in calibrator, control sample or analysed sample - react in the first step with a specific antibody coated on the walls of wells of a microtiter plate. After rinsing of unbound proteins follows next the second, incubation step, during which the specific antibody conjugated with an enzyme (horse-radish peroxidase) reacts with the coated thyrotropin. After incubation, the wells are rinsed and the conjugate bound in the wells is detected by the addition of a chromogenic substrate (TMB). The intensity of resulting colouration is proportional to the concentration of TSH in calibrators and analyzed samples.